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| Niu,Zhiwei; Wu,Siyu; Wu,Chongyang; Li,Na; Zhu,Haijing; Liu,Weishuai; Hua,Jinlian. |
| ABSTRACT Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis, during which unlimited spermatozoa is produced daily derived from SSCs in the testis throughout life of the male. Germline stem (GS) cells can be isolated from spermatogonia, which shared the characteristics of SSCs and embryonic stem cells (ESCs), and can be passaged stably in vitro. The study of GS cells contributes to understanding spermatogenesis process. However, little is known about the GS cells in domestic animals. Here, we report the successful establishment of a serum- and feeder-free system for multipotent male GS cells (mGSCs) from postnatal porcine testis. These cells expressed pluripotent markers, such as Oct4, Nanog, C-myc, and germline-specific markers... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Spermatogonial stem cells (SSCs); Multipotent male GS cells (mGSCs); Spermatid-like cells; Porcine. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100402 |
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